Born in Reynosa, Mexico and raised in Pasadena, Texas, Ana comes from a very close-knit and hardworking family, and is the younger of two daughters. She is married to Greg Luna, and they have a son, Gregory Eli Luna.

Ana graduated from Sam Rayburn High School in Pasadena, and received her Bachelor of Science in Political Science and Psychology from the University of Houston. Upon completion of her undergraduate studies, Ana attended the University of Texas School of Law. She received her Doctor of Jurisprudence in May of 2004.

In addition to representing District 143 in the Texas House of Representatives, she also practices law at Carrigan, McCloskey & Roberson, L.L.P. She is a member of various professional, civic and charitable organizations including the State Bar of Texas, a member of the Board of Directors for the Hispanic Bar Association of Houston, a Texas Bar Foundation Fellow, and she serves on the Board of Regents for the Texas Chiropractic College and Precinct2gether.

After the 82nd Legislative Session she received numerous awards and recognitions for her hard work including the "2011 Arrival Award" presented by the University of Houston School of Law, the Texas Association of Bilingual Educator's "Community Advocacy Award", and was honored as a champion for women's health by both Planned Parenthood and the American Congress of Obstetricians and Gynecologists (ACOG).